Coding
Part:BBa_K1923007:Design
Designed by: Tianyang Mao Group: iGEM16_Tsinghua (2016-10-14)
Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2394
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4673
Illegal XhoI site found at 220
Illegal XhoI site found at 567 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 139
Design Notes
Source
This part was assembled from previous BioBricks originating from distribution kits, and was integrated into a fusion protein.